Why we do serial dilution in microbiology

Serial dilution is a microbiological process where a substance is stepwise diluted in a solution. This serial dilution method is a technological method that is used in the isolation of microorganisms.

There are two types of dilution.

1. Simple dilution
2. Serial dilution.

Below is a discussion of what the serial dilution method is and how it’s to be calculated (1).

Let us see the basis of the solution, that is solute, and solvent before discussing serial dilution.

The solution is a mixture of two or more elements in a solvent.

It can be prepared by dissolving solutes in solvent or diluted in a diluent, e.g. water, alcohol, etc.

Serial dilution calculations microbiology

Usually, the same solvent or diluent is used for the dilution of the solution to prepare a working solution (1).

Serial dilution is a biotechnical method. In microbiology, isolation is defined as the separation of a strain from a natural or mixed population of living microorganisms.

Soil is a heterogeneous mixture of various types of microorganisms including bacteria, actinomycetes, fungi, algae, and protozoa.

These microorganisms

steps of serial dilution

how to do serial dilutions in biology